Western blotting is a core technique in molecular biology and cell biology, which is used to detect the presence of specific protein in a complex mixture extracted from a wide variety of biological samples, such as tissue homogenates or cultured cells. The intensities of the bands are displayed and the relative concentration of protein in the sample is quantified.Using rectangular selection tool, area around each bands to be analyzed are selected.Blot images are imported into the software and the contrast is adjusted in such a way to obtain a clear western blot image.The basic steps involved in densitometry analysis of proteins are as follows: It is basically a public domain Java image processing program that was developed at the National Institute of Health. In this section, the densitometry analysis, that is, the determination the variations in the density of protein samples by comparing it with a certain standard value/ determining the intensity of the specific bands in the western blot is done using the software Image J. A number of freely available online tools are currently available to analyze the biological images. The recent advances in the biological and medical field have made the image analysis increasingly important in this discipline. Visualization and image analysis methods are critical for understanding various features of cell biology, molecular biology and neuroscience applications. To compare the amount of protein in the given sample by analyzing the intensity of the band produced in the gel. ![]() In this experiment, image processing techniques are applied to study the western blot analysis.
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